Analysis of components of immune complexes in sera of patients with systemic lupus erythematosus
Okubo, Y.
Ryumachi 31(1): 36-44
1991
ISSN/ISBN: 0300-9157 PMID: 1857994 Document Number: 373877
The samples of immune complexes (IC) isolated through Clq tube from serum of SLE patients were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting for their antigens and antibodies. The isolated IC contained gamma, mu and L chain of immunoglobulin and various sizes of DNA ranging from 16KD to 55KD. The isolated IC showed antibodies reacting to 16KD DNA. In the isolated IC, DNAs with molecules less than 300 base pairs were detected. These DNA were double-stranded and hybridized with nuclear DNAs originated from human but not from other species.