Studies on the analysis of beta-blocking agents
Duan, H.J.; Fang, H.J.; Zhou, T.H.; Chen, C.
Yao Xue Xue Bao 26(5): 351-357
1991
ISSN/ISBN: 0513-4870 PMID: 1683506 Document Number: 373316
A method for the separation and identification of 13 beta-blockers using GC/MS and GC/NPD are described. The TFA and TMS derivatives were prepared by adding TFAA, MSTFA and MBTFA to the standard drugs. The mixture was heated at 65 degrees C for 20 min and chromatographed on FS capillary columns (HP-5). Preliminary results show that the derivatization with MSTFA is better than that with TFAA or MBTFA. There is not much difference between TFAA and MBTFA derivatization. The method is reliable, rapid and simple and may be used to screen these drugs and further applied to the analysis of biological fluids such as plasma and urine.