Use of high velocity mechanical injection for the transfer of foreign DNA into early mouse embryos

Zelenina, I.A.; Semenova, M.L.; Alimov, A.A.; Kolesnikov, V.A.; Golichenkov, V.A.; Zelenin, A.V.; Zelenina, I.A; Semenova, M.L; Alimov, A.A; Kolesnikov, V.A; Golichenkov, V.A; Zelenin, A.V

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Use of high velocity mechanical injection for the transfer of foreign DNA into early mouse embryos

Zelenina, I.A.; Semenova, M.L.; Alimov, A.A.; Kolesnikov, V.A.; Golichenkov, V.A.; Zelenin, A.V.

Genetika 27(12): 2182-2186

1991

ISSN/ISBN: 0016-6758

PMID: 1802800

Document Number: 372604

The possibility of high velocity mechanical transfer of foreign DNA into inner cell mass of mouse blastocyst was shown. Penetration of tungsten microparticles into early embryo cell nuclei and their localization on mitotic chromosomes was demonstrated. About 70% of developing embryos survived the bombardment. Total DNA of the mice born from bombarded embryos was analyzed by blot-hybridization and PCR with Southern hybridization. In three cases, the presence of the transferred plasmid DNA (pSV3-neo) was revealed.