Comparison of anti-RNA properties of normal and ellipticine functionalized alpha and beta-oligonucleotides
Lavignon, M.; Bertrand, J.R.; Porumb, H.; Rayner, B.; Imbach, J.L.; Paoletti, C.; Malvy, C.
Nucleic Acids Symposium Series 24: 117-119
1991
ISSN/ISBN: 0261-3166 PMID: 1668683 Document Number: 369631
RNA is not cleaved as a consequence of the binding of RNase H to the duplex between RNA and a complementary alpha-oligodeoxyribonucleotide (oligo). In consequence targets have been selected which do not a priori require the action of RNase H to inhibit genetic expression. Two models have been used: The Friend Murine Leukemia Virus (F-MuLV) and the synthesis of rabbit beta globin.alpha-oligos trigger specific inhibitions in both systems. The functionalisation in 5' with the intercalating agent 9-NH2-ellipticine renders the oligos resistant to degradation and allows a direct action on cells.