Immunohistochemical studies of ras oncogene product p21 in 7,12 dimethylbenz (a) anthracene-induced rat ovarian tumors
Kataoka, A.; Hirakawa, N.; Sugiyama, T.; Higashijima, T.; Nishida, T.; Yakushiji, M.
Nihon Sanka Fujinka Gakkai Zasshi 43(9): 1209-1213
1991
ISSN/ISBN: 0300-9165 PMID: 1919181 Document Number: 368390
In the present study, ras oncogen product p21 was analyzed immunohistochemically in rat ovarian tumors induced by 7,12 dimethylbenz (a) anthracene (DMBA). 1) After 28 .apprx. 38 (average 35.6) weeks, the tumors -6 adenomas, 30 adenocarcinomas, 3 sarcomase, one mixed mullerian tumor and 2 epidermal cysts- were produced in the rat ovaries. 2) The p21 positive reactions could be seen in the cytoplasm of the tumor cells. The positive rates were as follows: adenomas 83%, adenocarcinomas 57%, sarcomas 67%, mixed mullerian tumors 0%, epidermal cysts 100%, in which the positive reactions were obtained in squamous cells. Both of the serial-allografted tumor and DMBA-OC-1 were positive. 3) The present study showed that the ras oncogen is not a specific gene in ovarian sarcoma or in other tumors. 4) The present study has suggested that the ras oncogen plays a role in tumor genesis including benign tumors, by showing p21 positive in adenomas and epidermal cysts.