A new method for determination of nicotinic acid in plasma

Becker, B.; Hummel, K.

Arzneimittel-Forschung 40(5): 573-575


ISSN/ISBN: 0004-4172
PMID: 2383298
Document Number: 366611
A new and sensitive method for the detection of nicotinic acid in plasma is described. Human or rat plasma-samples from pharmacological or pharmacokinetic studies are lyophilized and extracted with methanol. After removal of the solvent the residue is converted with N-Methyl-N-trimethylsilyltrifluoracetamide to the trimethylsilylester of nicotinic acid, separated by vpc and detected with a mass selective detector via the fragment 180 amu. 6-Methyl-nicotinic acid is used as internal standard (fragment 194 amu). With this method a detection limit of 100 ng/ml plasma is reached.

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