Binding of [3H]cytochalasin B to tumoral islet cells
Garcia-Mateu, S.; Barreto, M.; Valverde, I.; Malaisse, W.J.
Biochemistry International 21(1): 69-75
1990
ISSN/ISBN: 0158-5231 PMID: 2167090 Document Number: 365640
Tumoral pancreatic islet cells of the RINm5F line are equipped with two classes of [3H]cytochalasin B binding sites with respective Kd of 0.4 and 7 microM. The binding of the fungal metabolite and its dissociation from the binding sites display rapid time courses. The binding is inhibited by D-glucose, more than by L-glucose, by phlorizin and by cytochalasin E. These findings are considered in the light of the dual action of cytochalasin B upon hexose transport and motile activity in islet cells.