Cystic fibrosis marker testing in Bohemia with polymerase chain reaction
Macek, M.; Böhm, I.; Arnold, L.; Smrt, J.; Duspivová, R.; Vávrová, V.; Macek, M.; Jelínková, E.; Schmidtke, J.
Acta Universitatis Carolinae. Medica 36(1-4): 108-111
1990
ISSN/ISBN: 0001-7116 PMID: 2130670 Document Number: 365616
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.