Selective suppression of polymerase chain reaction
Luk'ianov, K.A.; Gurskaia, N.G.; Bogdanova, E.A.; Luk'ianov, S.A.
Bioorganicheskaia Khimiia 25(3): 163-170
1999
ISSN/ISBN: 0132-3423 PMID: 10382038 Document Number: 498842
The selective suppression of the polymerase chain reaction and methods based upon it (construction of cDNA libraries from low amounts of biological material, subtractive hybridization and differential display of mRNA, fast cloning of full-size cDNA, chromosome walking, cloning in vitro, and others) are reviewed. These methods display a high effectiveness and, taken together, enable intricate DNA analyses to be performed--from the search for nontrivial sequences to the total sequencing of the corresponding genes.