Inhibition of translation of immunoglobulin mRNA in vitro using an alkylating oligonucleotide derivative
Vlasov, V.V.; Godovikov, A.A.; Zarytova, V.F.; Ivanova, E.M.; Nomokonova, N.Iu.
Molekuliarnaia Biologiia 24(1): 173-178
1990
ISSN/ISBN: 0026-8984 PMID: 2112224 Document Number: 363594
Effect of complementary oligonucleotides and their reactive derivatives on translation of mouse immunoglobulin G kappa light chain was investigated. It was found that oligonucleotide pTGCTCTGGTTT and shorter oligonucleotides complementary to the coding sequence of the mRNA (nucleotides 205-215) do not arrest translation of the mRNA in the rabbit reticulocyte cell-free translation system. Preincubation of the mRNA with the alkylating 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphamide derivative of the oligonucleotide completely suppresses the synthesis of the protein thus demonstrating higher efficiency of the reactive oligonucleotide derivatives as inhibitors of the mRNA function.