Single cell fluorescence photometry for quantification of antigens on alveolar macrophages
Pankow, W.; Neumann, K.; Rüschoff, J.; von Wichert, P.
Verhandlungen der Deutschen Gesellschaft für Pathologie 74: 253-257
1990
ISSN/ISBN: 0070-4113 PMID: 1708596 Document Number: 361750
The quantitation of cell surface antigens on alveolar macrophages (AM) using flow-cytometry is complicated by strong autofluorescence and wide variation of cell- size and autofluorescence. Therefore a microscope-fluorometric method was developed which allows simultaneous assessment of fluorescence of fluorochrome-labelled monoclonal antibodies and size in individual cells. Autofluorescence of smokers AM was found to be variable and considerably stronger than autofluorescence of nonsmokers AM. By means of a multi step determination of autofluorescence and specific fluorescence using different filter combinations of the microscope fluorometer density of membrane bound HLADR-antigens could be efficiently determined also in strongly autofluorescent smoker AM.