The histochemical detection of insulin in a culture of pancreatic endocrine tissue by using the pseudoisocyanine and immunofluorescence methods
Meĭramov, G.G.; Kohnert, K.D.; Turchin, I.S.; Tusupbekova, G.T.; Akhmetov, A.A.; Meĭramova, Z.G.
Problemy Endokrinologii 36(1): 66-69
1990
ISSN/ISBN: 0375-9660 PMID: 1691849 Document Number: 360383
Histochemical pseudoisocyanin and immunofluorescent methods were employed to investigate the level of insulin in cultivated pancreatic islet tissue (PIT) using two techniques: 1) staining of isolated PIT, cultivated on mica sheets; 2) staining of cultivated PIT sections. Stained sections ensure better results of microscopic and photometric investigations. On the whole, the most optimum method for histochemical analysis of the state of cultivated PIT is the combination of the above two techniques.