Detection of tubercle bacilli in clinical samples using genetic methods (PCR) compared with Lowenstein-Jensen culture methods
Boruń, M.; Rybczyńska, M.; Pawłowska, I.
Pneumonologia i Alergologia Polska 68(3-4): 131-137
2000
ISSN/ISBN: 0867-7077 PMID: 11004847 Document Number: 525202
The aim of this work was to compare data obtained using PCR assay for detection of Mycobacterium tuberculosis with culture. We report a test for detection of tubercle bacilli by PCR and identification at species level by capture plate hybridisation and enzyme-linked immunoassay. 222 clinical samples obtained from patients with confirmed and suspected tuberculosis were analysed. These specimens were tested parallelly by conventional culture on Löwenstein-Jensen slants and PCR test. For 205 samples a complete agreement between these methods was observed.