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Effect in vitro of propoxur on kinetics of K+ stimulated PNPPase and protection by thiol reagents

Babu, G.R.; Reddy, G.R.; Rajendra, W.; Chetty, C.S.

Biochemistry International 21(6): 1095-1104

1990

ISSN/ISBN: 0158-5231
PMID: 1964379
Document Number: 360244
Kinetics analysis of K+ stimulated PNPPase was studied in the rat brain synaptosomes in the presence of propoxur. Non-competitive inhibition with respect to activation by PNPP was seen by the decreased maximal velocity (Vmax) without change in Michaelis-Menten Constant (Km). Activation energy values (delta E) were increased suggesting the decreased catalytic potential of the enzyme. It is also observed that dithiothrietol (DTT) (76 microM), cysteine (82 microM) and glutathione (120 microM) neutralized the inhibition of K(+)-PNPPase by propoxur to different extents.

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