Kinetics of antibody and immunoglobulin-producing cells appearing in popliteal lymph nodes of mice stimulated with horseradish peroxidase
Ternynck, T.; Rodrigot, M.; Avrameas, S.
Journal of Immunology 119(4): 1321-1328
1977
ISSN/ISBN: 0022-1767 PMID: 561126 Document Number: 119835
Mice were given injections in their hind footpads of peroxidase emulsified in complete Freund's adjuvant. Kinetics of cells producing antibodies and cells producing immunoglobulins determinants or bromelain-treated mouse red cells. After a 2nd injection, Ab+ and Ig+Ab--producing cells appeared rapidly and simultaneously in approximately equal numbers; the peak number of Ab+-producing cells was seen on days 5-7 whereas the Ig+Ab--producing cells disappeared 3 days after challenge. The ratio of secreting vs. synthetizing cells remained constant throughout the response, around 2:10 for Ab+ cell population and 5:10 for Ig+Ab- cell population. With the immune cytoadherence test, cells forming rosettes with peroxidase-coated SRBC were not found in lymph nodes of unstimulation mice although they appeared 4 days after antigen injection. Their number increased exponentially until day 8 and then more slowly until day 14. Although after a secondary injection the number of rosette-forming cells was increased, the peak value attained only 20% of the peak value observed during the primary response. After injection of mice with peroxidase in complete Freund's adjuvant, the following apparently sequence occurs: appearance of an immunocyte population bearing peroxidase receptors; appearance of an immunocyte population synthesizing and secreting, or not, Ig without detectable antibody function, appearance of an immunocyte population synthetizing and secreting, or not, antibody.