A morphometric study of the effects of pressure on bone resorption in the middle ear of rats
Huang, C.C.; Yi, Z.X.; Yuan, Q.G.; Abramson, M.
American Journal of Otology 11(1): 39-43
1990
ISSN/ISBN: 0192-9763 PMID: 2407131 Document Number: 359155
Bone destruction is one of the clinical features of chronic otitis media with cholesteatoma. Bone resorption may be due to the epithelial debris accumulated in the cholesteatoma epidermal sac that acts as foreign body material inducing a destructive granulation tissue and creates pressure on the bony middle ear. This compressive force then induces bone resorption. The present study was designed to replicate certain conditions similar to those in cholesteatoma leading to bone resorption. Four types of materials were implanted into the middle ear cavity of rats: (1) laminaria, in expandable seaweed material, (2) preswollen laminaria, (3) keratin powder suspension, and (4) surgical grade silicone, which when bent exerts pressure on the bulla wall. The placement of laminaria segments in the middle ear cavity of rats was followed by swelling of the implanted materials within 7 days. The bulla bone response was by neoosteogenesis as well as active bone resorption. The new bone was observed on the external and/or internal surface of the tympanic bone. The cochlear bone also showed extensive bone resorption in the animals. Osteogenesis was rarely observed on the capsule of the cochlea. We also observed no bone resorption at sites without presence of inflammatory connective tissue between laminaria and bone. The typical multinucleated osteoclasts were often seen at the resorption area but the majority of bone resorption sites were characterized by the presence of mononuclear cells and other inflammatory cells. Preswollen laminaria, keratin powder, and silicone strips induced minimal bone resorption. No resorption was observed in the bony cochleas of these experimental groups. The morphometric quantitative analysis of bone resorption in each group was analyzed through 10-.mu. serial sections of bone using a computerized digitizer system with an IBM microcomputer PS/2 and a Sigma scan program. The volumes of bone resorption were measured by the sum of areas for each section and multiplied by the thickness of sections. We found that (1) bone resorption in the group with laminaria was significantly greater than the group implanted with preswollen laminaria, keratin, and silicone strips; (2) bone resorption in the bulla was not statistically different for the groups even though they underwent tests for varying time periods; and (3) the extent of bone resorption was not significantly different between rats implanted with preswollen laminaria, keratin, and silicone strips. These findings further suggest that pressure as well as foreign body material can cause bone resorption, and pressure associated with a foreign body produces greater bone resorption than either condition on its own.