Neoplastic cell spreading in rodent transplantable tumor models. II. Santamaria tumor (syngeneic keratinizing squamous cell carcinoma)
Roveta, G.
Bollettino della Societa Italiana di Biologia Sperimentale 66(3): 295-302
1990
ISSN/ISBN: 0037-8771 PMID: 2378738 Document Number: 358638
A syngeneic transplantable tumor was obtained in our laboratory by inducing a skin squamous cell carcinoma in BALB/c mice treated with benzo(a) pyrene and UVA. Single tumor cell suspensions obtained by finely disrupted tumor masses were either i.p. or intramuscularly injected and developed (100% takes) invasive tumors maintaining in subsequent analogue serial transplantations identical histopathological aspects. Many Grunwald Giemsa stained organ imprints of tumor bearing mice showed disseminated tumor cells as well as a number of infiltrating host defense cells (principally neutrophils) despite the mice were in a terminal status. Many Grunwald Giemsa stained cryostat sections showed numerous mast cells lining the invasive tumor front and allowed to detect in the liver tumor cells migrated from primary tumor (localized in femoral muscle) adhering to endothelial cells may be to perform extravasation.