Top Abstract Purchase Related
Home
>
Node
>
Subnode

Qualitative and quantitative histochemical evaluation of the dehydrogenase activity in human glioblastoma cells treated with beta-interferon

Nano, R.; Rezzani, R.; Zibera, C.; Gibelli, N.; Bacciocchi, G.

Bollettino della Societa Italiana di Biologia Sperimentale 66(3): 279-285

1990

ISSN/ISBN: 0037-8771
PMID: 2378736
Document Number: 357127
In an attempt to clarify the role of beta interferon in vitro cell systems, the metabolic expression of dihydrofolate reductase (DHFR), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), by histochemical methods was studied. DHFR was also quantified by flow cytometry. Glioblastoma cell line with or without beta interferon was used. Three days after the treatment the DHFR reaction was much less intense than in the control. Quantitative data confirmed these results. Immediately afterwards, most cells exhibited much more intense reaction. These facts underlined that, in this biological model in vitro, beta interferon could reduce the synthesis of these enzymes only for a short period.

Document emailed within 1 workday
Secure & encrypted payments

Schmidt, W.; Gabler, W.; Wendler, D. 1970: Quantitative evaluation of the histochemical succinate dehydrogenase demonstration and its dependence upon different reaction conditions Acta Histochemica 37(2): 250-258
Dashev, G.; Stankusheva, T. 1972: Histochemical studies on the enzyme activity of 3-beta-ol-dehydrogenase in the adrenal cortex in hypercorticism Eksperimentalna Meditsina i Morfologiia 11(2): 102-107
Nagao, S.; Nakano, A.; Moritake, K.; Morikawa, S.; Harada, T. 1997: Synergistic antitumor effects of human interferon-beta and interferon-gamma on human gastric adenocarcinoma cells Human Cell 10(1): 95-104
Furuhata, T.; Kawakami, M.; Okita, K.; Kimura, Y.; Kihara, C.; Tsuruma, T.; Ohmura, T.; Yamaguchi, K.; Hata, F.; Katsuramaki, T.; Sasaki, K.; Hirata, K. 2006: Plasma level of a 5-fluorouracil metabolite, fluoro-beta-alanine correlates with dihydropyrimidine dehydrogenase activity of peripheral blood mononuclear cells in 5-fluorouracil treated patients Journal of Experimental and Clinical Cancer Research: Cr 25(1): 79-82
Orlova, T.G.; Zhdanova, L.V.; Mentkevich, L.M.; Shcheglovitova, O.N.; Solov'ev, V.D. 1979: Interferon-producing activity of bone marrow cells treated with mRNA for interferon when transplanted to irradiated mice Voprosy Virusologii 5: 541-544
Hahn, J.U.; Scholer, H.F. 1983: Qualitative und quantitative Röntgenanalyse von Schwermetallen in Sedimenten im REM durch Röntgenfluorescenz-Anregung. II: Anwendung - Analyse aux RX qualitative et quantitative de métaux lourds dans les sédiments par fluorescence RX en microscopie électronique à balayage. II. Application - Determination of heavy metals in sediments by X-ray fluorescence in SEM (qualitative and quantitative evaluation). II. Application Fresenius' Zeitschrift für Analytische Chemie 315(8): 683-686
Shimada, Y.; Shimoyama, M. 1984: Growth-inhibitory activity of human recombinant beta-interferon (GKT-beta) in vitro Gan 75(12): 1116-1124
Speirs, V.; Jenkins, S.; White, M.C. 1993: Growth factor regulation of 17 beta-hydroxysteroid dehydrogenase activity in a human ovarian cell line: modulation by 17 beta-estradiol Anticancer Research 13(5a): 1399-1403
Hatziagapiou, K.; Braoudaki, M.; Karpusas, M.; Tzortzatou, F.-Stathopoulou 2011: Evaluation of Antitumor activity of Gefitinib in Pediatric Glioblastoma and Neuroblastoma Cells Clinical Laboratory 57(9-10): 781-784
Nagai, N. 1985: Electron microscopic study on the structure and the function of the granulosa cell in the human ovary--ultrastructure and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity Nihon Sanka Fujinka Gakkai Zasshi 37(6): 871-878
Ouyang, X.; Qu, Y.; Liu, S.; Liu, B. 1998: Qualitative and quantitative analyses of telomerase activity in cultured cells Hua Xi Yi Ke da Xue Xue Bao 29(3): 244-247; 255
Press, R.D.; Misra, A.; Gillaspy, G.; Samols, D.; Goldthwait, D.A. 1989: Control of the expression of c-sis mRNA in human glioblastoma cells by phorbol ester and transforming growth factor beta 1 Cancer Research 49(11): 2914-2920
Vera, O.; Johannin, G.; Muñoz, G.; Roffi, J. 1994: Quantitative histo-enzymological study of delta 5-3 beta-hydroxysteroid dehydrogenase activity in rat testis during the perinatal period. Relation with testicular crisis in the newborn rat Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 188(4): 355-362
Brotchi, J. 1975: Primary and secondary glioblastoma. Distinction by glutamate dehydrogenase activity Acta Neurologica Belgica 75(3-4): 134-142
Macarini, L.; Marini, S.; Pietrapertosa, A.; Scardapane, A.; Ettorre, G.C. 2005: Non cardiopatic and cardiopatic beta thalassemic patients: quantitative and qualitative cardiac iron deposition evaluation with MRi La Radiologia Medica 109(1-2): 77-90
Labuda, T.; Kugler, P. 1988: Quantitative histochemical study of the NAD-dependent isocitrate dehydrogenase in the hippocampus of the NZB mouse Zeitschrift für Mikroskopisch-Anatomische Forschung 102(5): 865-878
Yamamoto, K.; Mizutani, Y.; Nakanishi, H.; Fujiwara, J.; Ishida, H.; Toiyama, D.; Abe, K.; Hayashi, I.; Okada, K.; Kawauchi, A.; Mizuno, M.; Yoshida, J.; Miki, T. 2008: Significant antitumor activity of cationic multilamellar liposomes containing human interferon-beta gene in combination with 5-fluorouracil against human renal cell carcinoma International Journal of Oncology 33(3): 565-571
Begemann, M.; Kashimawo, S.A.; Heitjan, D.F.; Schiff, P.B.; Bruce, J.N.; Weinstein, I.B. 1998: Treatment of human glioblastoma cells with the staurosporine derivative CGP 41251 inhibits CDC2 and CDK2 kinase activity and increases radiation sensitivity Anticancer Research 18(4a): 2275-2282
Krasnov, I.B.; Il'ina-Kakueva, E.I. 1970: Technic of freezing animal tissue at -155 degrees for quantitative and qualitative histochemical study Tsitologiia 12(2): 262-264
Esterbauer, H.; Nöhammer, G.; Schauenstein, E.; Weber, P. 1973: Quantitative histochemical demonstration of sulfhydrylgroups by the DDD-staining-method. 3. Quantitative cytospectrometrical determination in Ehrlich-ascites-tumour cells Acta Histochemica 47(1): 106-114