Isolation of concanavalin A-reactive low-molecular weight allergen from house dust
Mitra, I.; Chatterjee, B.P.
Indian Journal of Biochemistry and Biophysics 27(1): 28-32
1990
ISSN/ISBN: 0301-1208 PMID: 2341162 Document Number: 356308
A concanavalin A-reactive low-molecular weight (MW approximately 7000) glycoprotein allergen has been isolated from house dust. The crude allergen was fractionated by Sephadex gel filtration followed by separation into two allergenic components by affinity chromatography on concanavalin A-Sepharose column. The column-bound fraction contains Man (11.7%), Gal (2.34%), GlcNAc (3.47%) and a trace of Glc and has been proved to be more potent allergen for house dust-sensitive patients both in vivo and in vitro tests.