Quantitative determination of the carbohydrate determinants of O-antigens of Yersinia pseudotuberculosis and Pseudomonas fluorescens
Naberezhnykh, G.A.; Solov'eva, T.F.; Ovodov, I.S.
Bioorganicheskaia Khimiia 16(8): 1089-1095
1990
ISSN/ISBN: 0132-3423 PMID: 1704703 Document Number: 355393
Interactions of O-specific polysaccharides, LPS and LPS-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and monovalent Fab-fragments of the antibodies were investigated. The association constants and quantity of Fab-fragments bound to these antigens were established. The interaction of the LPS and LPS-protein complexes was shown to depend considerably on concentration of antigens, and only 10-15% of carbohydrate determinants are accessible for binding with antibodies.