Calculation of kinetic parameters of a reversible enzymatic reaction in states of maximal activity
Heinrich, R.; Hoffmann, E.; Holzhütter, H.G.
Biomedica Biochimica Acta 49(8-9): 891-902
1990
ISSN/ISBN: 0232-766X PMID: 2082929 Document Number: 353707
Optimal values of the rate constants of a reversible enzymatic reaction are calculated by maximizing the enzymatic activity. The results depend on the substrate and product concentrations. Expressions for optimal maximal activities are derived and conclusions are drawn for the optimal ratios of substrate concentrations and Michaelis-constants.
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