Ultramicroassay of red cell group using Terasaki-plate
Kuwao, F.; Mizobuchi, N.; Takeda, I.; Horimi, T.; Takahashi, I.
Rinsho Byori. Japanese Journal of Clinical Pathology 38(2): 213-219
1990
ISSN/ISBN: 0047-1860 PMID: 2329723 Document Number: 350343
In order to simplify the testing-method of blood groups (ABO, Rh, MN, Ss, P, I, Lewis, Duffy, Kidd and Diego) and the assay of glycosyl transferases activity, we have developed a new method using Terasaki plate (T-plate method), and examined its usefulness in the routine examination. As results followings were obtained: 1) The sensitivity of T-plate method was equal or superior than that of the common method using test-tube. 2) Samples used for T-plate method were smaller in volume compared with those for the common tube test method. 3) The percent of coincidence between T-plate method and tube test method was 100% for ABO, Rh, MN, Ss, P, I, Duffy, Kidd and Diego grouping. They were 98% for Lewis(a) and 96% for Lewis(b) grouping, respectively. These results indicate the superiority of T-plate method than the common method using test-tubes in respects of smaller volume of samples, lower price and handling of a large amount of samples.