Interaction of chlorpromazine with hemoglobin

Bhattacharyya, M.; Chaudhuri, U.; Poddar, R.K.

Indian Journal of Biochemistry and Biophysics 27(4): 240-244

1990


ISSN/ISBN: 0301-1208
PMID: 2286389
Document Number: 349395
Binding of chlorpromazine (CPZ), a widely used antidepressant tranquilizer, with hemoglobin has been studied by equilibrium dialysis method. r/Cf versus r plot was typically concave downwards revealing the positive cooperative nature of binding. Binding parameters, namely the affinity constant (K) and the degree of cooperativity (nH) were determined from the Hill plot. Oxygen was found to be released gradually from hemoglobin with gradual addition of CPZ, the extent of oxygen release depending on the stoichiometric ratio of CPZ: hemoglobin (D/P).

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