Inhibition of PAF-induced histamine secretion and bronchoconstriction by WEB 2086
Chand, N.; Pillar, J.; Nolan, K.; Diamantis, W.; Sofia, R.D.
Research Communications in Chemical Pathology and Pharmacology 67(1): 155-158
1990
ISSN/ISBN: 0034-5164 PMID: 1691522 Document Number: 349071
Platelet activating factor (PAF) stimulates histamine secretion from rabbit mixed leukocytes in a concentration-dependent fashion (EC50 10 ng/ml). The PAF (10 ng/ml)-induced histamine secretion was inhibited by WEB 2086 (IC50 = 20 nM). Furthermore, PAF (10 ng/kg, i.v.)-induced bronchoconstriction in guinea pig was also inhibited by WEB 2086 (ID50 = 5.3 micrograms/kg, i.v., 5 minutes). These data showed the existence of PAF-receptors on rabbit basophils and in guinea pig airways which are highly sensitive to blockade by WEB 2086.