Effects of some protein-reactive compounds on K+ flux into mitochondria
Diwan, J.J.; Moore, C.
Biochemistry International 22(5): 843-848
1990
ISSN/ISBN: 0158-5231 PMID: 2099149 Document Number: 348017
The pathway of unidirectional K+ flux into respiring mitochondria is sensitive to the protein reactive compounds mersalyl and dicyclohexylcarbodiimide (DCCD). When treated with either of these reagents, mitochondria retain sensitivity to other reagents which affect K+ flux into untreated mitochondria. The present studies show that the K+ influx mechanism modified by pretreatment with DCCD remains sensitive to inhibition by quinine. K+ influx stimulated by mersalyl, in the absence of exogenous Ca++, retains sensitivity to inhibition by quinine and to some extent by Mg++. The results support the conclusion that K+ uptake by mitochondria modified by mersalyl or DCCD occurs via the same proteinaceous pathway as that which mediates K+ uptake by untreated mitochondria.