In vitro reaction between methylene blue and the chromosomal DNA of E. coli
Izbirak, A.
Mikrobiyoloji Bulteni 23(4): 348-355
1989
ISSN/ISBN: 0374-9096 PMID: 2488943 Document Number: 345424
In the present work, the chromosomal DNA isolated from the bacterium E. coli was treated with methylene blue. Formation of a heat-stable complex with methylene blue and E. coli chromosomal DNA, has changed the hyperchromic characteristics of E. coli DNA to some extent. It seemed possible that in vivo binding of the dye with DNA may inhibit the separation of DNA chains thus preventing the DNA replication.