A significance of dual parameter flow cytometric DNA analysis using the anti-keratin antibody
Ishikawa, H.; Tagawa, Y.; Miyashita, K.; Yokota, M.; Yasutake, T.; Okada, D.; Kunisaki, T.; Tomita, M.
Human Cell 2(1): 70-73
1989
ISSN/ISBN: 0914-7470 PMID: 2485104 Document Number: 345187
Flow cytometric DNA analysis using the anti-cytokeratin antibody was carried out in order to estimate more reliable measurement in single cell suspension obtained from solid tumors. It was difficult to detect a DNA aneuploidy with DI of 2.0 by one parameter analysis of DNA. Whereas it could be detected easily by using dual parameter analysis of cytokeratin and DNA. And also, the pattern of DNA multiploidy could be selected for cytokeratin positive cell population by gate analysis.