Flow cytometric analysis of a marine LAS-degrading consortia
López-Amorós, R.; Comas, J.; Garcia, M.T.; Vives-Rego, J.
Microbios 101(398): 23-36
2000
ISSN/ISBN: 0026-2633 PMID: 10677841 Document Number: 522130
The specific nucleic acid fluorochrome SYTO-13 was used in flow cytometric analysis to assess changes in the density and heterogeneity of marine bacterial populations which biodegrade linear alkylbenzene sulphonate (LAS). Seawater samples with LAS and incubated in the laboratory (20 degrees C, 100 rpm, 30 days) were used to monitor LAS-degrading consortia. Flow cytometric studies and culture methods were used to characterize the LAS degrading bacterioplankton consortia. Fluorescence and scatter signals enabled us to define three regions (R1, R2 and R3) in the dual parameter cytograms. The distribution of the bacterial counts in these regions allowed us to monitor the formation and evolution of the consortia.