An alpha-satellite DNA sequence, alpha-RI-6, specific for human chromosomes 13 and 21, detected using the RFLP technic with digoxigenin labelled probes
Mazura, I.; Brdicka, R.; Srám, R.J.; Jurov, J.; Kapras, J.
Casopis Lekaru Ceskych 128(40): 1269-1271
1989
ISSN/ISBN: 0008-7335 PMID: 2573427 Document Number: 344521
The authors compared two at present most widely used techniques for labelling DNA probes: a) radioactive labelling by means of the radioisotope 32P; non-radioactive labelling using the hapten digoxigenin for the visualization of the hybridization process on nylon membranes. Then sensitivity of the technique of non-radioactive labelling of heterochromatin probes was equivalent to the radioactive method.
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