3H-thymidine incorporation by interstitial myocardial cells in the rat with a single administration of the label
Zamaraeva, E.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 108(12): 739-741
1989
ISSN/ISBN: 0365-9615 PMID: 2634455 Document Number: 338315
The hearts were taken 1, 2, 3, 4, 5, 6, 7, 14 and 21 days after a single injection of 3H-thymidine into 20 young male Wistar rats. Label incorporation was determined on half-thin slices derived through the whole left ventricular wall. First labeled nuclei are seen on the day 1 at the level of 0.4 +/- 0.08%, their quantity reaches maximum (1.66 +/- 0.1%) on day 4 and declines to 0.51 +/- 0.1% at day 7. On day 21 0.1 +/- 0.14% cells contained radioactivity. Maximal label concentration was found in middle intramural layer (2.44 +/- 0.24% on day 4), and reached only 1.36 +/- 0.36% in subendocardial and 1.16 +/- 0.24% in subepicardial layer. These data allow us to suppose the existence of linkage between interstitial cells repopulation and functional activity of different myocardial layers, and also the existence of short living and long living fibroblast subpopulations.