Production of rheumatoid factor-like substance and arthritic findings in knees of mice immunized with E. coli 0:14
Itoh, J.
Nihon Seikeigeka Gakkai Zasshi 63(5): 569-579
1989
ISSN/ISBN: 0021-5325 PMID: 2677173 Document Number: 335377
Four strains of mice (A. SW, B10. D2, C57BL/6, DBA/1J) were immunized by subcutaneous injections of heat-killed E. coli 0: 14. After single or multiple immunizations, histologic sections of knee joints and a serum rheumatoid factor-like substance (RFLS) at different time intervals were studied. Immunized A. SW. mice developed hyperplasia of synovial lining cells (25 out of 49 knees) and pannus (9 out of 49 knees) which was significantly higher rate than control. A serum rheumatoid factor-like substance (RFLS) was detected in B10. D2 and C57BL/6 mice by using RAHA test and enzyme-linked immunosorbent assay. Serum IgM-RFLS was positive in all B10. D2 mice (11 of 11 mice) immunized for 24 weeks. The incidence of serum IgM-RFLS positive mice in C57BL/6 immunized for 40 weeks (3 of 4 mice) was significantly higher than in control (p less than 0.05). Production of serum RFLS was not recognized in a single-immunized group of B10. D2 mice on statistical evaluation. These findings provided evidence that arthritis was developed in multiple-immunized groups of A. SW mice and RFLS was produced in multiple-immunized groups of B10. D2 and C57BL/6 mice. In addition, these findings suggested that multiple-immunization was necessary for the production of RFLS.