Dialysable specific transfer factor in mice immunized with attenuated Langat virus from the tick-borne encephalitis complex: generation, action and quantitative assay
Mayer, V.; Gajdosová, E.; Valásková, M.; Gombosová, A.; Oravec, C.
Acta Virologica 26(6): 453-465
1982
ISSN/ISBN: 0001-723X PMID: 6188353 Document Number: 186053
Cytolytic T lymphocyte assay was developed in order to measure the response of inbred C3H mice to dialyzable specific transfer factor (STF), induced in subadult outbred mice by 1 shot immunization with the attenuated Langat virus. The first STF activity in mouse splenic leukocytes was detected 48-72 h after virus administration. The conversion of splenic T-cell cytotoxic response in C3H mice in vivo occurred 15-21 h after STF administration. The killing activity of T cells, induced by STF, given prior to the live virus, augmented the specific cytolytic T-cell response. In the live virus-primed mice the booster effect was markedly enhanced when administration of STF preceded the 2nd immunization dose. In the serum of STF recipients, interferon was irregularly detected attaining low levels for short time periods. Temperatures of 56.degree. C for 60 min abolished the activity of at least 104 murine STF units, temperatures of 37.degree. C lowered this activity by 3 log10 units after 24 h. Chromatography of the dialyzed leukocyte lysate on Sephadex G-25 column usually yielded 5 peaks. The 2nd peak showed an increased content of ribose-bound and protein materials and, as a rule, a relatively concentrated STF activity.