Spectrotypic analysis of humoral response in human hydatidosis
D'Amelio, R.; Del Giudice, G.; De Rosa, F.; Brighouse, G.; Teggi, A.; Lambert, P.H.
Clinical and Experimental Immunology 76(1): 117-120
1989
ISSN/ISBN: 0009-9104 PMID: 2786776 Document Number: 334845
The spectrotyptic patterns of anti-Echinococcus granulosus and anti-F(ab')2 antibodies were analysed in 13 patients with different forms of hydatidosis by means of isoelectric focusing (IEF). Specific anti-echinococcus antibodies and specific echinococcus antigens were obtained by crossed affinity chromatography between patient serum and cyst fluid, as previously described (D'Amelio et al., 1985). A wide spectrotype in the pattern of anti-Echinococcus antibodies was observed, with strong homologies among the different sera mainly at acid and neutral pH. One serum displayed faint, nearly inapparent bands. Conversely, the pattern of anti-F(ab')2 antibodies in the six patients in whom they were positive was limited but heterogeneous, ranging from monoclonal through oligoclonal to polyclonal. A predominant clone was identified in the patient with a polyclonal pattern. A correlation between qualitative IEF spectrotypic patterns and the quantitative results of immunoenzymatic assays was observed for both types of antibodies.