Construction of chickpea genomic library
Koundal, K.R.; Mehta, S.L.; Sharma, R.P.
Indian Journal of Experimental Biology 27(10): 858-860
1989
ISSN/ISBN: 0019-5189 PMID: 2635143 Document Number: 334538
For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.
Document emailed within 1 workday
Related Documents
Dubrovskaia, V.V.; Ulitin, A.B.; Laman, A.G.; Gileva, I.P.; Bormotov, N.I.; Il'ichev, A.A.; Brovko, F.A.; Shchelkunov, S.N.; Belanov, E.F.; Tikunova, N.V. 2007: Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus Molekuliarnaia Biologiia 41(1): 173-185Jitsurong, S.; Chirathaworn, C.; Brown, N.F.; Beacham, I.R.; Tumwasorn, S. 2003: Searching for virulence Burkholderia pseudomallei genes by immunoscreening the lambda ZAPII expressed genomic library Southeast Asian Journal of Tropical Medicine and Public Health 34(4): 810-821
Zabarovskiĭ, E.R.; Allikmets, R.L. 1985: Construction of a gene library using partial filling of DNA sticky ends Bioorganicheskaia Khimiia 11(6): 849-852
Sharma, P.; Lönneborg, A.; Stougaard, P. 1993: PCR-based construction of subtractive cDNA library using magnetic beads Biotechniques 15(4): 610 612
Chen, Y.; Yang, N.; Hong, Y. 1996: Construction of gene library of attenuated liver hepatitis A vaccine (H2 strain) Zhonghua Yi Xue Za Zhi 76(5): 342-344
Bhattacharyya, S.; Mandal, R.K. 1999: Cloning and sequencing of an apparently recombinant promoter for napin gene from Brassica campestris genomic library and its evolutionary significance Indian Journal of Biochemistry and Biophysics 36(4): 221-226
Murakami, H.; Hohsaka, T.; Sisido, M. 2000: Random insertion and deletion mutagenesis for construction of protein library containing nonnatural amino acids Nucleic Acids Symposium Series 2000(44): 69-70
Zhou, X.; Yao, L.; Yu, S. 2001: NIES-90 microcystin producing algae strain genome library construction and one isolated gene analysis Zhonghua Liu Xing Bing Xue Za Zhi 22(4): 290-292
Zhang, Y-xin.; Liu, Y.; Liu, K-tai.; Cheng, J.; Chen, X-hua.; Wang, G.; Wang, G-quan. 2003: Construction of subtracted cDNA library in human Jurkat T cell line induced by arsenic trioxide in vitro Zhonghua Yu Fang Yi Xue Za Zhi 37(6): 403-407
Oliĭnyk, O.S.; Kaberniuk, A.A.; Redchuk, T.A.; Korotkevych, N.V.; Labyntsev, A.Iu.; Romaniuk, S.I.; Kolybo, D.V.; Komisarenko, S.V. 2009: Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies specific to diphtheria toxin B subunit Ukrains'kyi Biokhimichnyi Zhurnal 81(2): 68-79
Ma, X-Li.; Wang, B-Bei.; Wu, P.; Lu, Y-Ping.; Zhou, J-Feng.; Ma, D. 2009: Construction of anti-sense cDNA library of human breast cancer cells during apoptosis induced by trichostatin A and preliminary screening of essential genes Zhonghua Yi Xue Za Zhi 89(7): 480-484
Prombona, A.; Subramanian, A.R. 1991: Construction of a chloroplast DNA library from rye (Secale cereale), a cereal plant of temperature-inducible chloroplast ribosome deficiency Biochemistry International 24(3): 559-566
Xu, H.J.; Zhou, H.Y.; Hu, S.X.; Pan, X.R. 1989: Cloning of apoprotein AI gene and observation on DNA polymorphism of apoprotein AI/CIII gene-loci in genomic library from a liver of Chinese Chinese Medical Journal 102(3): 169-173
Womboh, B.S.H. 1993: The library and information resources of the University of Agriculture, Makurdi Library, Nigeria: a self-study Quarterly Bulletin of the International Association of Agricultural Information Specialists 38(1): 9-15
Pizer, I.H.; Franz, D.R.; Brodman, E. 1963: Mechanization of library procedures in the medium-sized medical library. I. The serial record Bulletin of the Medical Library Association 51: 313-338
Vannucci, L.; Frigenti, L.; Faussone-Pellegrini, M-Simonetta. 2011: From conception to birth: ancient library sources of embryology and women anatomy kept in the Biblioteca Biomedica of the Università degli Studi di Firenze (Biomedical Library of Florence University) Italian Journal of Anatomy and Embryology 116(2): 93-103
Miura, N.; Kucho, K-Ichi.; Noguchi, M.; Miyoshi, N.; Uchiumi, T.; Kawaguchi, H.; Tanimoto, A. 2014: Comparison of the genomic sequence of the microminipig, a novel breed of swine, with the genomic database for conventional pig In Vivo 28(6): 1107-1111
Shimoyama, T.; Sasaki, T. 2007: Genomic and post-genomic approaches to cancer biomarker discovery - microarray standards at last Gan to Kagaku Ryoho. Cancer and ChemoTherapy 34(10): 1527-1534
Weber-Hall, S.; Anderson, J.; McManus, A.; Abe, S.; Nojima, T.; Pinkerton, R.; Pritchard-Jones, K.; Shipley, J. 1996: Gains, losses, and amplification of genomic material in rhabdomyosarcoma analyzed by comparative genomic hybridization Cancer Research 56(14): 3220-3224
Ghaffari, S.H.; Lobb, C.J. 1993: Structure and genomic organization of immunoglobulin light chain in the channel catfish. An unusual genomic organizational pattern of segmental genes Journal of Immunology 151(12): 6900-6912