Infectivity studies on anopheles stephensi using Plasmodium cynomolgi B infection in rhesus monkeys
Puri, S.K.; Kamboj, K.K.; Dutta, G.P.
Tropical Medicine and Parasitology Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit 40(4): 409-411
1989
ISSN/ISBN: 0177-2392 PMID: 2623421 Document Number: 333980
The characteristics of primary and secondary asexual peak parasitaemia during sporozoite-induced P. cynomolgi bastianelli infections in 40 Macaca mulatta were studied. Colony bred A. stephensi NICD strain were fed on different days on the gametocyte carrying monkeys and the infectivity of mosquitoes as determined by oocyst count on day 8 post-feeding was recorded. Following the day of sporozoite inoculation, the mean prepatent period was 8.58 +or- 0.87 days. The primary asexual peak was attained on day 8.38 +or- 2.27 and the secondary peak on day 15.15 +or- 2.63 after patency. The infectivity rate was 100% in 144 batches of mosquitoes fed 3 days before to 3 days after the secondary asexual peak. Infective gametocyte peak coincided with the secondary asexual peak parasitaemia, with the results that maximum oocyst number was observed in batches fed on the day of secondary peak or one day prior to or one day after the secondary peak. Infectivity during the period of primary asexual peak was inconsistent. Mosquito infectivity results showed that the ideal time for an infective blood meal was 12-18 days after patency. The characteristics of primary and secondary asexual peak parasitaemia during sporozoite induced Plasmodium cynomolgi B infections in 40 rhesus monkeys have been studied. Colony bred Anopheles stephensi were fed on different days on the gametocyte carrying monkeys and the infectivity of mosquitoes as determined by oocyst count on day 8 post-feeding was recorded. Following the day of sporozoite inoculation, the mean prepatent period was 8.58 +or- 0.87 days. The primary asexual peak was attained on day 8.38 +or- 2.27 and the secondary peak on day 15.15 +or- 2.63 after patency. Infectivity rate was 100% in 144 batches of mosquitoes fed three days before to three days after the secondary asexual peak. Judging from the day of patency, the high oocyst count was obtained between day 12-18 after patency. Infective gametocyte peak coincided with the secondary asexual peak parasitaemia, with the result that maximum oocyst number was observed in batches fed on the day of secondary peak or one day prior to or one day after the secondary peak. Infectivity during the period of primary asexual peak was inconsistent.AS.