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A study of the interaction of substrates with cytochrome P-450 by a method of UV- and 1H-NMR spectroscopy

Vol'dman, I.I.; Guliaeva, L.F.; Vaĭner, L.M.; Liakhovich, V.V.

Bioorganicheskaia Khimiia 15(8): 1044-1055

1989

ISSN/ISBN: 0132-3423
PMID: 2590249
Document Number: 333487
Acceleration of substrate longitudinal relaxation (T1) was used to study cytochrome P-450-aminopyrine (1st type substrate) and P-450-4-methoxypyridine (2nd type substrate) complexes. Dissociation constant, T1 and/or residence time of substrate in the complex can be obtained from the dependence of T1 of substrate protons on substrate concentration. Basing on the relaxation times, distances between Fe3+ ion in the active site and protons of the substrate moiety were determined. For aminopyrine all the distances proved to be about 8 A. In the P-450-4-methoxypyridine complex the pyridine nitrogen is directed towards Fe3+ ion. Cytochrome P-450 is compared with its denatured form, cytochrome P-420, and metmyoglobin.

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Kharatian, S.A.; Kaiushin, L.P. 1982: ESR study of interaction between adenylate kinase, substrates and Mn2+ ions Biofizika 27(1): 10-13
Grishanova, A.Iu.; Mishin, V.M.; Liakhovich, V.V. 1985: Interaction between cytochrome P-448 and NADP-cytochrome P-450 reductase in reconstituted microsomal membranes Biokhimiia 50(3): 369-374
Wainwright, P.; Power, D.M.; Thomas, E.W.; Davies, J.V. 1978: The interaction of cytochrome C with phospholipids. A pulse-radiolysis study International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 33(2): 151-162
Mank, V.V.; Sotskova, T.Z.; Derevianko, A.I.; Kurilenko, O.D. 1977: Study of the interaction of water with cholesterol in organic media by the methods of dielectric and NMR spectroscopy Biofizika 22(5): 794-800
McLean, M.A.; Sligar, S.G. 1995: Thermodynamic characterization of the interaction between cytochrome b5 and cytochrome c Biochemical and Biophysical Research Communications 215(1): 316-320
Evtushenko, A.D. 1972: Use of electrophoresis for concentration and extraction of agents of intestinal infections from various substrates. I. Experimental elaboration of a method of electrophoretic extraction of Sh. sonnei and S. typhi from substrates artificially seeded with different quantities of cells Laboratornoe Delo 10: 616-618
Smith, L.; Bolgiano, B.; Davies, H.C. 1988: Kinetics of the interaction of cytochrome c oxidase of Paracoccus denitrificans with Paracoccus and mitochondrial cytochrome c Progress in Clinical and Biological Research 274: 619-635
Khadzhiev, K.K.; Chirko, I.G. 1980: Oxidative phosphorylation of Krebs cycle substrates in experimental treatment of enterocolitis with cytochrome c Voprosy Meditsinskoi Khimii 26(2): 154-157
Itou, T.; Nojiri, M.; Ootsuka, Y.; Nakamura, K. 2006: Study of the interaction between hair protein and organic acid that improves hair-set durability by near-infrared spectroscopy Journal of Cosmetic Science 57(2): 139-151
Braginskaia, F.I.; Sadykhova, S.K.; Zaretskiĭ, A.A.; Musaev, N. 1974: Study of polyriboadenylic acid (polyrA) in aqueous solutions by the method of ultrasonic absorption spectroscopy Biofizika 19(6): 987-991
Irshaid, Y.; Branch, R.A.; Adedoyin, A. 1996: Metabolic interactions of putative cytochrome P4503A substrates with alternative pathways of dapsone metabolism in human liver microsomes Drug Metabolism and Disposition: the Biological Fate of Chemicals 24(2): 164-171
Bhagavathi, R.; Devi, S.A. 1993: Interaction of exercise and age on substrates of carbohydrate metabolism Indian Journal of Experimental Biology 31(1): 72-75
Odinokov, S.E.; Nabiullin, A.A.; Glazunov, V.P.; Kurika, A.V.; Pavlenko, A.F. 1985: Study of the spatial structure of the protein component of the carcino-embryonic antigen by circular dichroism, Raman spectroscopy and UV-spectroscopy Bioorganicheskaia Khimiia 11(10): 1315-1322
Adamovich, T.V.; Pikuleva, I.A.; Usanov, S.A.; Chashchin, V.L. 1989: Study of the role of lysine residues of the cholesterol hydroxylating cytochrome P-450 by a method of chemical modification Biokhimiia 54(7): 1206-1216
Gorshkova, I.I.; Lavrik, O.I.; Filippov, V.V. 1981: Role of the carboxylic groups in interaction of phenylalanyl-tRNA synthetase with substrates Molekuliarnaia Biologiia 15(1): 62-71
Shashkova, E.A.; Vikha, I.V.; Vichutinskiĭ, A.A.; Khorlin, A.I. 1970: Interaction of lysozyme with low-molecular inhibitors and modified substrates containing beta-(1-6)-glucosaminidic bonds Biokhimiia 35(1): 124-131
Lankin, V.Z.; Gordeeva, N.T.; Tikhaze, A.K.; Vikhert, A.M. 1985: Animal lipoxygenases. the nature of substrates and changes in conformation of reticulocyte lipoxygenase in its interaction with membranes Biokhimiia 50(11): 1894-1900
Sellers, C. 1990: Combined sample preparation and inductively coupled plasma emission spectroscopy method for determination of 23 elements in solid wastes: summary of collaborative study Journal - Association of Official Analytical Chemists 73(3): 476-478
Sípal, Z.; Anzenbacher, P.; Putz, Z.; Chlumský, J.; Krivanová, O. 1979: Interaction of cytochrome P-450 with hydrocarbons Acta Biologica et Medica Germanica 38(2-3): 483-494
Kinoshita, N.; Yamamura, T.; Teranuma, H.; Katayama, T.; Tamanyu, M.; Negoro, T.; Satoh, K.; Sakagami, H. 2002: Interaction between dental metals and antioxidants, assessed by cytotoxicity assay and ESR spectroscopy Anticancer Research 22(6c): 4017-4022