ESR study of interaction between adenylate kinase, substrates and Mn2+ ions
Kharatian, S.A.; Kaiushin, L.P.
Biofizika 27(1): 10-13
1982
ISSN/ISBN: 0006-3029 PMID: 6279168 Document Number: 184392
Plant adenylate kinase was first investigated by ESR technique at room temperature. In contrast to previous studies the measurement conditions corresponded to maximal enzyme activity. It was shown that adenylate kinase addition provoked Mn2+--adenine nucleotide binary complex distribution and rearrangement of components with the formation of ternary Mn2+--adenine nucleotide--adenylate kinase complex. The same results were obtained in the studies on myokinase from rabbit muscle.