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Efficiency of Micro Sort flow cytometry for producing sperm populations enriched in X- or Y-chromosome haplotypes: a blind trial assessed by double and triple colour fluorescent in-situ hybridization

Vidal, F.; Fugger, E.F.; Blanco, J.; Keyvanfar, K.; Català, V.; Norton, M.; Hazelrigg, W.B.; Black, S.H.; Levinson, G.; Egozcue, J.; Schulman, J.D.

Human Reproduction 13(2): 308-312

1998

ISSN/ISBN: 0268-1161
PMID: 9557828
DOI: 10.1093/humrep/13.2.308
Document Number: 330606
Using fluorescent in-situ hybridization (FISH) we have evaluated, on a blind basis, the efficiency of flow cytometry to separate human X- and Y-chromosome bearing spermatozoa. Our data demonstrate that human spermatozoa can be sorted to a purity of 80-90% for X spermatozoa and of 60-70% for Y spermatozoa. Our results using triple FISH fully agree with the sorting treatment used in each case and corroborate the efficiency of the flow sorting technique for sperm sex selection. In these limited samples (200-500 sperm/donor), the frequencies of disomic or diploid spermatozoa were not increased when comparing the sorted samples with unselected samples or with our control series.

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Mahdy, E.; Yoshihiro, S.; Zech, L.; Wester, K.; Pan, Y.; Busch, C.; Döhner, H.; Kallioniemi, O.; Bergerheim, U.; Malmström, P.U. 1999: Comparison of comparative genomic hybridization, fluorescence in situ hybridization and flow cytometry in urinary bladder cancer Anticancer Research 19(1a): 7-12
Mark, H.F.; Mills, D.R.; Kim, E.; Santoro, K.; Quddus, M.R.; Lathrop, J.C. 1996: Fluorescent in situ hybridization assessment of chromosome copy number in buccal mucosal cells Cytobios 87(349): 117-126
Gilladoga, A.D.; Edelhoff, S.; Blackwood, E.M.; Eisenman, R.N.; Disteche, C.M. 1992: Mapping of MAX to human chromosome 14 and mouse chromosome 12 by in situ hybridization Oncogene 7(6): 1249-1251
Michalová, K. 1995: Fluorescent in situ hybridization in clinical cytogenetics Casopis Lekaru Ceskych 134(3): 73-76
Domracheva, E.V.; Kobzev, I.N. 1997: The use of fluorescent in situ hybridization (FISH) in chronic myeloleukemia Terapevticheskii Arkhiv 69(7): 71-74
Gole, L.A.; Bongso, A. 1997: Fluorescent in-situ hybridization--some of its applications in clinical cytogenetics Singapore Medical Journal 38(11): 497-503
Spadoro, J.P.; Payne, H.; Lee, Y.; Rosenstraus, M.J. 1990: Single copies of HIV proviral DNA detected by fluorescent in situ hybridization Biotechniques 9(2): 186-195
Stepanov, V.A.; Puzyrev, V.P. 2000: Haplotypes of two diallelic Y chromosome loci in the indigenous and migrant populations of Siberia Genetika 36(1): 87-92
Savostikova, M.V.; Volchenko, N.N.; Rusakov, I.G.; Borisova, O.V.; Golovashchenko, M.P. 2011: The fluorescent hybridization in situ as applied in diagnostics of urinary bladder cancer Klinicheskaia Laboratornaia Diagnostika 8: 38-40
Mark, H.F.; Mills, D.R.; Santoro, K.; Quddus, M.R.; Lathrop, J.C. 1997: Fluorescent in situ hybridization analysis of cervical smears. A pilot study of 20 cases Annals of Clinical and Laboratory Science 27(3): 224-229
Chaddha, V.; Agarwal, S.; Phadke, S.R.; Halder, A. 2003: Low level of mosaicism in atypical Prader Willi syndrome: detection using fluorescent in situ hybridization Indian Pediatrics 40(2): 166-168
Baurmann, H.; Cherif, D.; Berger, R. 1993: Interphase cytogenetics by fluorescent in situ hybridization (FISH) for characterization of monosomy-7-associated myeloid disorders Leukemia 7(3): 384-391
Sarasophona, S.; Promsawat, K.; Rerkamnuaychok, B. 2000: Identification of sex chromosome by fluorescence in situ hybridization Journal of the Medical Association of Thailand 83(Suppl 1): S89-S94
Cheng, L.; Bucana, C.D.; Wei, Q. 1996: Fluorescence in situ hybridization method for measuring transfection efficiency Biotechniques 21(3): 486-491
Beverloo, H.B.; Le Coniat, M.; Wijsman, J.; Lillington, D.M.; Bernard, O.; de Klein, A.; van Wering, E.; Welborn, J.; Young, B.D.; Hagemeijer, A. 1995: Breakpoint heterogeneity in t(10;11) translocation in AML-M4/M5 resulting in fusion of AF10 and MLL is resolved by fluorescent in situ hybridization analysis Cancer Research 55(19): 4220-4224
Regan, J.M.; Oldenburg, P.S.; Park, H.D.; Harrington, G.W.; Noguera, D.R. 2003: Simultaneous determination of bacterial viability and identity in biofilms using ethidium monoazide and fluorescent in situ hybridization Water Science and Technology: a Journal of the International Association on Water Pollution Research 47(5): 123-128
Huang, Y.; Sun, X.; Li, Q. 1999: Diagnosis of sex chromosome abnormality by fluorescence in-situ hybridization Zhonghua Yi Xue Za Zhi 79(2): 106-108
Dallenbach-Hellweg, G.; Dallenbach, F.D.; Sievers, S. 1979: Microscopic, micro-chemical and fluorescent optical findings in endometrium with a copper-T intrauterine device in situ Geburtshilfe und Frauenheilkunde 39(7): 575-579
Wise, R.A.; Malamet, R.; Wigley, F.M. 1987: Acute effects of nifedipine on digital blood flow in human subjects with Raynaud's phenomenon: a double blind placebo controlled trial Journal of Rheumatology 14(2): 278-283
Eckschlager, T.; McClain, K. 1996: Comparison of fluorescent in situ hybridization (FISH) and the polymerase chain reaction (PCR) for detection of residual neuroblastoma cells Neoplasma 43(5): 301-303