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Fluorescent in situ hybridization in clinical cytogenetics

Michalová, K.

Casopis Lekaru Ceskych 134(3): 73-76

1995

ISSN/ISBN: 0008-7335
PMID: 7712529
Document Number: 453351
During the last few years molecular biology methods expanded into human cytogenetics. This is in close connection with advanced technologies of DNA probes preparation and possibilities of their non-radioactive labelling by means of enzymatic incorporation of modified nucleotides as well as their hybridization with complementary DNA of chromosomes and interphase nuclei. FISH became a useful method in the clinical research. We present the short review of FISH methodologies and their applications for studies of translocation, deletions, amplifications and other chromosomal rearrangements in genetic and oncologic patients. The sensitivity of these methods is approximately 1-10 kb and therefore precise localization of genes on chromosomes is possible. Except gene mapping FISH can be used for comparative genomic mapping (CGH) and for identification of chromosomal changes of tumor cells.

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Baurmann, H.; Cherif, D.; Berger, R. 1993: Interphase cytogenetics by fluorescent in situ hybridization (FISH) for characterization of monosomy-7-associated myeloid disorders Leukemia 7(3): 384-391
Glassman, A.B. 1998: Cytogenetics, in situ hybridization and molecular approaches in the diagnosis of cancer Annals of Clinical and Laboratory Science 28(6): 324-330
Virmani, A.K.; Tonk, V.S.; Gazdar, A.F. 1998: Comparison between fluorescence in situ hybridization and classical cytogenetics in human tumors Anticancer Research 18(3a): 1351-1356
Solé, F.; Espinet, B.; Woessner, S. 1998: Priority of using conventional cytogenetics, in situ hybridization and PCR in the study of hematologic neoplasms Sangre 43(1): 3-5
Domracheva, E.V.; Kobzev, I.N. 1997: The use of fluorescent in situ hybridization (FISH) in chronic myeloleukemia Terapevticheskii Arkhiv 69(7): 71-74
Spadoro, J.P.; Payne, H.; Lee, Y.; Rosenstraus, M.J. 1990: Single copies of HIV proviral DNA detected by fluorescent in situ hybridization Biotechniques 9(2): 186-195
Poddighe, P.J.; Moesker, O.; Smeets, D.; Awwad, B.H.; Ramaekers, F.C.; Hopman, A.H. 1991: Interphase cytogenetics of hematological cancer: comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes Cancer Research 51(7): 1959-1967
Savostikova, M.V.; Volchenko, N.N.; Rusakov, I.G.; Borisova, O.V.; Golovashchenko, M.P. 2011: The fluorescent hybridization in situ as applied in diagnostics of urinary bladder cancer Klinicheskaia Laboratornaia Diagnostika 8: 38-40
Mark, H.F.; Mills, D.R.; Santoro, K.; Quddus, M.R.; Lathrop, J.C. 1997: Fluorescent in situ hybridization analysis of cervical smears. A pilot study of 20 cases Annals of Clinical and Laboratory Science 27(3): 224-229
Mark, H.F.; Mills, D.R.; Kim, E.; Santoro, K.; Quddus, M.R.; Lathrop, J.C. 1996: Fluorescent in situ hybridization assessment of chromosome copy number in buccal mucosal cells Cytobios 87(349): 117-126
Chaddha, V.; Agarwal, S.; Phadke, S.R.; Halder, A. 2003: Low level of mosaicism in atypical Prader Willi syndrome: detection using fluorescent in situ hybridization Indian Pediatrics 40(2): 166-168
Beverloo, H.B.; Le Coniat, M.; Wijsman, J.; Lillington, D.M.; Bernard, O.; de Klein, A.; van Wering, E.; Welborn, J.; Young, B.D.; Hagemeijer, A. 1995: Breakpoint heterogeneity in t(10;11) translocation in AML-M4/M5 resulting in fusion of AF10 and MLL is resolved by fluorescent in situ hybridization analysis Cancer Research 55(19): 4220-4224
Regan, J.M.; Oldenburg, P.S.; Park, H.D.; Harrington, G.W.; Noguera, D.R. 2003: Simultaneous determination of bacterial viability and identity in biofilms using ethidium monoazide and fluorescent in situ hybridization Water Science and Technology: a Journal of the International Association on Water Pollution Research 47(5): 123-128
Eckschlager, T.; McClain, K. 1996: Comparison of fluorescent in situ hybridization (FISH) and the polymerase chain reaction (PCR) for detection of residual neuroblastoma cells Neoplasma 43(5): 301-303
Choi, Y.J. 1990: In situ hybridization using a biotinylated DNA probe on formalin-fixed liver biopsies with hepatitis B virus infections: in situ hybridization superior to immunochemistry Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 3(3): 343-347
Arranz, E.; Robledo, M.; Martínez, B.; Prieto, E.; Román, A.; Benítez, J. 1997: Detection of t(15;17) using cytogenetic, fluorescent in situ hybridization, and molecular techniques. Comparative study of 11 patients with acute promyelocytic leukemia Sangre 42(1): 11-15
Kawai, H.; Kaneko, S.; Kobayashi, K. 1995: Detection of hepatitis C virus (HCV) infected cell using in situ hybridization and PCR in situ hybridization Nihon Rinsho. Japanese Journal of Clinical Medicine 53 Suppl (Part 1): 307-311
Vinogradova, O.A.; Savchenko, V.G.; Neverova, A.L.; Diachenko, L.V.; Domracheva, E.V.; Liubimova, L.S.; Mendeleeva, L.P. 2001: Study of the time course of mixed chimerism by fluorescent in situ hybridization in patients with chronic myeloid leukemia after allogenic transplantation of bone marrow Terapevticheskii Arkhiv 73(7): 26-34
Sidoni, A.; Ferri, I.; Cavaliere, A.; Bellezza, G.; Scheibel, M.; Bucciarelli, E. 2006: Detection of HER-2/neu (c-erbB-2) overexpression and amplification in breast carcinomas with ambiguous immunohistochemical results. A further contribution to defining the role of fluorescent in situ hybridization Anticancer Research 26(3b): 2333-2337