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Synthesis and properties of RNA with catalytic activity

Odai, O.; Sakata, T.; Orita, M.; Hiroaki, H.; Uesugi, S.; Tanaka, T.

Nucleic Acids Symposium Series 21: 105-106

1989

ISSN/ISBN: 0261-3166
PMID: 2608453
Document Number: 329830
Ribooligonucleotides, which form the self-cleavage domain of a satellite RNA of tobacco ringspot virus, were synthesized by the solid-phase phosphoramidite method using o-nitrobenzyl groups for 2'-hydroxyl protection. The oligomers were obtained in quantities sufficient for NMR measurement. Specific cleavage at an expected site was observed when the three RNA fragments were mixed in the presence of magnesium ions.

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