Histone electrophoretic pattern in the characterization of synaptonemal complexes
Vilardell, J.; Coll, M.D.; Querol, E.; Egozcue, J.
Cellular and Molecular Biology 35(2): 207-214
1989
ISSN/ISBN: 0145-5680 PMID: 2731196 Document Number: 329167
The presence of a stoichiometric electrophoresis pattern of histones in carefully isolated synaptonemal complexes is reported. The use of this pattern is suggested as an internal standard of synaptonemal complex purification, in addition to the more generally used electron micrographs. This is especially useful in experiments leading to the characterization of the protein components of SCs. The use of mice of an age at which pachytenes predominate (90%) in the prophase-cell population is also advantageous to improve the final yield in synaptonemal complexes.