The role of protein phosphorylation in mast cell secretion, stimulated by concanavalin A. Connection to the cytoskeleton
Dulin, N.O.; Goncharenko, E.N.; Kudriashov, I.B.; Graevskaia, E.E.; Kravtsov, G.M.
Biokhimiia 54(9): 1428-1433
1989
ISSN/ISBN: 0320-9725 PMID: 2590681 Document Number: 328931
The role of cytoskeleton and protein phosphorylation in concanavalin A and phorbol ester (PMA) induced mast cell secretion was investigated. It was shown that the receptor coupled with lectin interacts with the cytoskeleton. When the ligand-receptor complex is formed, an increased phosphorylation of some proteins is induced. The same proteins are phosphorylated under the influence of PMA, a protein kinase C activator, thus suggesting that protein kinase C is involved in the regulation of mast cell exocytosis. The results obtained testify to the effect that the mechanism of mast cell degranulation induced by concanavalin A is due to modification of the cytoskeleton.