Subclass distribution and IgE responses after treatment in human schistosomiasis
Evengård, B.; Hammarström, L.; Smith, C.I.; Johansson, S.G.; Linder, E.
Clinical and Experimental Immunology 73(3): 383-388
1988
ISSN/ISBN: 0009-9104 PMID: 3208450 Document Number: 325784
The IgG and IgA subclass distribution of specific antibodies as well as the distribution of total and specific IgE in 15 patients with schistosomiasis (including 9 with confirmed Schistosoma mansoni and one with S. haematobium infection) was determined in consecutive samples before and after initiation of treatment. Oxamniquine was given to 11 patients (including all those with S. mansoni infection) at 30 mg/kg, praziquantel was given to the patient infected with S. haematobium at 40-50 mg/kg, one patient received metrifonate at 7-10 mg/kg repeated after 14 days and praziquantel, and 2 received oxamniquine and praziquantel. An adult S. mansoni antigen and a S. mansoni SEA were used as antigens in the ELISA. After initiation of treatment a rise was noted in certain subclasses and a correlation was found for specific IgG1 and IgG4 serum levels in the egg-excreting patients against adult worm antigen and for specific IgG4 and IgE levels in sera from the 8 patients with chronic disease. They also had a rise of the specific IgA1 titre and 6 of them also of specific IgA2. Numbers of eosinophilic granulocytes reached a peak after 2 weeks in 7 of the 8 patients. The increase of eosinophils was an early event as opposed to the incidence of peak of the determined specific isotypes. The associated rise in IgG1, IgG4 and IgE antibody concentrations and eosophils may suggest a causal relationship, possibly induced by common interleukins. The IgG and IgA subclass distribution of specific antibodies as well as the distribution of total and specific IgE in 15 patients with schistosomiasis of eosinophilic granulocytes reached a peak after 2 weeks in 7 of the 8 patients. The increase of eosinophils was an early event as opposed to the incidence of peak of the determined specific isotypes. The associated rise in IgG1, IgG4 and IgE antibody concentrations and eosinophils may suggest a causal relation possibly induced by common interleukins.AS.