Immune responses during human schistosomiasis mansoni. II. Occurrence of eosinophil-dependent cytotoxic antibodies in relation to intensity and duration of infection
Sher, A.; Butterworth, A.E.; Colley, D.G.; Cook, J.A.; Freeman, G.L.; Jordan, P.
American Journal of Tropical Medicine and Hygiene 26(5 Pt 1): 909-916
1977
ISSN/ISBN: 0002-9637 PMID: 907053 Document Number: 120340
Plasma samples from St. Lucians were tested for the presence of antibodies which co-operate in vitro with normal human leucocytes in causing cytotoxic damage to schistosomula of Schistosoma mansoni. The in vitro antibody activity, which has been previously shown to depend on eosinophil effector cells was detected in 56% of the individuals with known, current S. mansoni infections and in 14% of control subjects from the same endemic area. Quantitatively, eosinophil dependent cytotoxic antibody (EDCA) activity, when expressed as the maximum amount of damage to schistosomula induced at high plasma concentration, correlated significantly with the intensity of S. mansoni infection as determined by faecal egg count, the highest levels of activity occurring in patients with stool counts of 60 eggs/ml or greater. In addition, plasma EDCA activity was found to correlate with the in vitro blastogenic responsiveness of patients' lymphocytes to 3 different parasite antigen preparations. In contrast, titrations of EDCA activity failed to reveal a relationship between EDCA titre and the most recent egg count performed on each subject. However, a significant correlation was observed when titres were compared to egg counts averaged over a 3-year period. Neither maximal EDCA activity nor titre was found to correlate with the duration of known schistosome infection. [AS].