Phosphoprotein phosphatase nonspecifically hydrolyzes CoA
Reziapkin, V.I.; Moiseenok, A.G.
Ukrainskii Biokhimicheskii Zhurnal 60(5): 74-77
1988
ISSN/ISBN: 0201-8470 PMID: 2849829 Document Number: 325282
CoA hydrolysis was studied by a homogenous phosphoprotein phosphatase (EC 3.1 3.16) preparation from bovine spleen nuclei at pH 5.8. Phosphoprotein phosphatase catalyzed hydrolysis of the CoA 3'-phosphoester bond to form dephospho-CoA and Pi. The Km value for phosphoprotein phosphatase with CoA as substrate was 3.7 mM, the specific activity - 0.26 mmol Pi.min-1.mg-1. Phosphoprotein phosphatase did not essentially catalyze the calcium pantothenate hydrolysis (not more than 2% as compared with the CoA hydrolysis rate).