Radioreceptor method of determination of opioid peptides in the rat brain
Davletov, E.G.; Kurochkin, I.N.; Zaĭtsev, S.V.
Voprosy Meditsinskoi Khimii 34(4): 136-138
1988
ISSN/ISBN: 0042-8809 PMID: 2848361 Document Number: 324980
A procedure is described for quantitative radioreceptor estimation of opioid peptides in rat brain using lyophilized membrane preparations. As opiate receptors exhibit high rate of heterogeneity estimation of delta- and mu-opioid activities was carried out by means of displacing of 3H-DADLE and 3H-DAGO, respectively. Content of opioid peptides in brain of preadolescent rats was studied during experimental burn trauma using the procedure developed. An increase in the delta- and mu-opioid activities was found in rat brain tissue within one day after thermal burns.