1,25-Dihydroxyvitamin D stimulates sodium-dependent phosphate transport by renal outer cortical brush-border membrane vesicles by directly affecting membrane fluidity
Suzuki, M.; Kawaguchi, Y.; Momose, M.; Morita, T.; Yokoyama, K.; Miyahara, T.
Biochemical and Biophysical Research Communications 150(3): 1193-1198
1988
ISSN/ISBN: 0006-291X PMID: 3342065 Document Number: 323093
We examined the effects of several forms of vitamin D added to renal brush-border membrane suspensions on phosphate and glucose transport and on membrane fluidity. The 1,25-D stimulated and the other vitamin D decreased phosphate uptake. In contrast, glucose uptake was not affected by the treatment of vitamin D. The 1,25-D resulted in a significant shift of the lower transition temperature in Arrhenius plots for phosphate, but not for glucose uptakes, from 15.degree.C to 11.5.degree.C. These data indicate that the 1,25-D may alter membrane fluidity, limited to the phosphate transporter, thus affecting the phosphate uptake.