Transport of 5-oxoproline into rabbit renal brush border membrane vesicles
Ganapathy, V.; Roesel, R.A.; Leibach, F.H.
Biochemical and Biophysical Research Communications 105(1): 28-35
1982
ISSN/ISBN: 0006-291X PMID: 7092855 Document Number: 189984
5-Oxoproline is transported intact into rabbit renal brush border membrane vesicles by a Na+-gradient dependent mechanism. The transport occurs against a concentration gradient. The equilibrium transport of 5-oxoproline decreases with increasing medium osmolarity. The predominant portion of transport occurs into an osmotically responsive intravesicular space, the non-specific surface binding being negligible. Competition experiments show that transport of 5-oxoproline is inhibited by most of the neutral amino acids whereas acidic, basic and imino acids do not have any effect.