Morphologic evidence that disruption of the Golgi apparatus in PFHR 9-tumor cells causes disturbance in the traffic through this organelle
Junqueira, L.C.; Kaminskas, R.; Levi, J.E.; Joazeiro, P.P.; Montes, G.S.
Journal of Submicroscopic Cytology and Pathology 20(2): 263-268
1988
ISSN/ISBN: 1122-9497 PMID: 3395965 Document Number: 322110
PFHR 9 in a murine teratocarcinoma-derived tumor which produces basement membrane components. Electron microscopy of the tumor cells disclosed a disorganiztion of the Golgi complex whose saccules, instead of being flattened, round up into 0.13 .mu.m-wide spherical vesicles (SV). Their shape does not permit the normal sticking of parallel cisternae, and thus these SV become intermingled with transport vesicles (TV) which measure 44 nm in diameter. The cytoplasm of these tumor cells contains numerous multivesiclular bodies (MVB) of varying size (ranging from 0l.3 to 2.6 .mu.m), which occupy 7% of the cellular volume. These MVB are packed with very many vesicles similar in all aspects to the TV, and also contain a few larger vesicles which resemble the altered Golgi saccules (SV). Since these MVB display morphologic evidence for gradual lysis of their contents and strongly react with the cytochemical accumulation of TV. This seems to be a unique example of a disease of the Golgi stack, with consequent accumulation and disposal of this material via autophagic vacuoles.