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The antiglucocorticoid RU486 downregulates the expression of interleukin-2 receptors in normal human lymphocytes

Antonakis, N.; Markogiannakis, E.; Theodoropoulou, M.; Georgoulias, V.; Stournaras, C.; Gravanis, A.

Journal of Steroid Biochemistry and Molecular Biology 39(6): 929-935

1991

ISSN/ISBN: 0960-0760
PMID: 1751392
DOI: 10.1016/0960-0760(91)90351-5
Document Number: 318041
The effects of RU-486, which has anti-glucocorticoid properties, on expression of the interleukin-2 receptors (IL-2R) in human lymphocytes were examined in terms of its dose effect on 2 types of binding sites, and its suppression of beta-chain IL-2R messenger RNA production. Human peripheral lymphocytes stimulated with phytohemagglutinin (PHA) were incubated with the synthetic glucocorticoid dexamethasone and with RU-486 at various doses. Synthesis of 2 types of IL-2R was measured by immunofluorescence assay of the alpha chain. RU-486 inhibits in a dose- dependent fashion the expression of both classes of IL-2R, acting like an agonist. Maximal suppression was seen at a concentration of 10 mcM for the low affinity binding site, and at 1 mcM for the high affinity rate. Messenger RNA was determined by extracting RNA, fractioning it by electrophoresis on agarose gel, and hybridizing with cDNA probes inserted with a plasmid. RU-486 significantly lowered the accumulation of beta-chain IL-2R mRNA transcripts. Thus RU-486 at pharmacological concentrations acts like a glucocorticoid agonist with immunosuppressive effects.

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Kierszenbaum, F.; Lopez, H.M.; Sztein, M.B. 1996: Trypanosoma cruzi downregulates the production of interleukin-2, interferon-gamma, interleukin-4 and interleukin-5 by activated human lymphocytes Journal of Parasitology 82(4): 652-656
Airoldi, I.; Guglielmino, R.; Carra, G.; Corcione, A.; Gerosa, F.; Taborelli, G.; Trinchieri, G.; Pistoia, V. 2002: The interleukin-12 and interleukin-12 receptor system in normal and transformed human B lymphocytes Haematologica 87(4): 434-442
Rullas, J.ín.; Bermejo, M.; García-Pérez, J.; Beltán, M.; González, N.; Hezareh, M.; Brown, S.J.; Alcamí, J.é 2004: Prostratin induces HIV activation and downregulates HIV receptors in peripheral blood lymphocytes Antiviral Therapy 9(4): 545-554
Sahraoui, Y.; Allouche, M.; Perrakis, M.; Clemenceau, C.; Jasmin, C.; Georgoulias, V. 1992: Phorbol myristate acetate-induced expression of high-affinity interleukin 2 receptors and production of interleukin 2 by human acute lymphoblastic leukemia T cells Leukemia 6(4): 295-303
D'Onofrio, M.; de Grazia, U.; Morrone, S.; Cuomo, L.; Spinsanti, P.; Frati, L.; Gulino, A.; Ragona, G. 2000: Expression of neurotrophin receptors in normal and malignant B lymphocytes European Cytokine Network 11(2): 283-291
Feldman, R.D.; Hunninghake, G.W.; McArdle, W.L. 1987: Beta-adrenergic-receptor-mediated suppression of interleukin 2 receptors in human lymphocytes Journal of Immunology 139(10): 3355-3359
Itoh, K.; Kumagai, K. 1980: Effect of tunicamycin and neuraminidase on the expression of Fc-IgM and -IgG receptors on human lymphocytes Journal of Immunology 124(4): 1830-1836
Myc, A.; DeAngelis, P.; Lassota, P.; Melamed, M.R.; Darzynkiewicz, Z. 1991: Effect of swainsonine on interleukin-2 alpha chain receptor expression and proliferation of human lymphocytes Clinical and Experimental Immunology 84(3): 406-410
Mitiushova, E.V.; Shatrova, A.N.; Zenin, V.V.; Aksenov, N.D.; Marakhova, I.I. 2013: The STAT5 signaling in the expression of alpha-subunit of interleukin-2 receptor in human blood lymphocytes Tsitologiia 55(6): 421-429
Kaplan, D.R.; Braciale, V.L.; Braciale, T.J. 1984: Antigen-dependent regulation of interleukin 2 receptor expression on cloned human cytotoxic T lymphocytes Journal of Immunology 133(4): 1966-1969
Carpentieri, U.; Kalmaz, G.; Ezell, E.; Thorpe, L.W. 1988: Trace metals, surface receptors and growth of human normal and leukemic lymphocytes Anticancer Research 8(6): 1393-1397
Damle, N.K.; Doyle, L.V.; Bender, J.R.; Bradley, E.C. 1987: Interleukin 2-activated human lymphocytes exhibit enhanced adhesion to normal vascular endothelial cells and cause their lysis Journal of Immunology 138(6): 1779-1785
Oreshkin, A.E.; Gudkova, M.V.; Miasishcheva, N.V. 1992: The expression of plasmalemma transcobalamin-Ii receptors on human blood lymphocytes stimulated by mitogens Biulleten' Eksperimental'noi Biologii i Meditsiny 114(8): 185-187
Li, D.Q.; Kuang, A.K.; Ding, T. 1989: Nuclear 3,5,3'-triiodothyronine receptors in circulating human lymphocytes in normal, hyper-and hypothyroid subjects Zhonghua Nei Ke Za Zhi 28(5): 286-289; 315
Ryffel, B.; Müller, S.; Foxwell, B. 1987: Cyclosporine a allows the expression of high affinity interleukin 2 binding sites on anti-T-cell antibody activated human T lymphocytes Transplantation Proceedings 19(1 Part 2): 1199-1201
Zeng, H.; Zhang, X.; Chen, Z. 1998: Expression and significance of interleukin 6, interleukin 8 and their receptors in acute promyelocytic leukemia during all-trans retinoic acid induction treatment Zhonghua Zhong Liu Za Zhi 20(5): 357-360
Huleihel, M.; Maymon, E.; Piura, B.; Prinsloo, I.; Benharroch, D.; Yanai-Inbar, I.; Glezerman, M. 1997: Distinct patterns of expression of interleukin-1 alpha and beta by normal and cancerous human ovarian tissues European Cytokine Network 8(2): 179-187
Sun, A.; Chu, C.T.; Liu, B.Y.; Wang, J.T.; Leu, J.S.; Chiang, C.P. 2000: Expression of interleukin-2 receptor by activated peripheral blood lymphocytes upregulated by the plasma level of interleukin-2 in patients with recurrent aphthous ulcers Proceedings of the National Science Council Republic of China. Part B Life Sciences 24(3): 116-122
Gao, B.-a.; Xiong, W.-n.; Xu, Y.-j.; Zhang, Z.-x.; Cao, Y.; Tang, Y.-j.; Ye, T.; Du, C.-l. 2006: Effects of recombinant adeno-virus vector carrying interleukin-5 antisense on the expression of interleukin-5 mRNA and protein in CD4+ T lymphocytes of asthmatic rats Zhonghua Nei Ke Za Zhi 45(4): 298-301
Nyman, T.; Pekonen, F. 1993: The expression of insulin-like growth factors and their binding proteins in normal human lymphocytes Acta Endocrinologica 128(2): 168-172